中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (34): 5423-5428.doi: 10.3969/j.issn.2095-4344.2014.34.003

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

京尼平交联Ⅰ型胶原蛋白材料与人脂肪间充质干细胞的生物相容性

王 刚1，亢 婷1，刘 毅2，刘刚强1   

  1. 1兰州大学第二临床医学院，甘肃省兰州市  730030；2解放军兰州军区兰州总医院全军烧伤整形外科中心，甘肃省兰州市 730050
  • 修回日期:2014-07-13 出版日期:2014-08-20 发布日期:2014-08-20
  • 通讯作者: 刘毅，教授，主任医师，研究生导师，解放军兰州军区兰州总医院全军烧伤整形外科中心，甘肃省兰州市 730050
  • 作者简介:王刚，男，1981年生，河南省荥阳市人，汉族，2006年西安交通大学医学院毕业，医师，主要从事脂肪组织工程研究。
  • 基金资助:

    全军医学科研“十二五”重点课题(BWS11C061)，课题名称：用于战创伤软组织缺损修复材料与移植装置的研究

Biocompatibility of Genipin cross-linked type I collagen with human adipose-derived stem cells in vitro

Wang Gang1, Kang Ting1, Liu Yi2, Liu Gang-qiang1   

  1. 1Second Clinical College of Lanzhou University, Lanzhou 730030, Gansu Province, China; 2Military Center for Burn and Plastic Surgery, Lanzhou General Hospital of Lanzhou Military Region, Lanzhou 730050, Gansu Province, China
  • Revised:2014-07-13 Online:2014-08-20 Published:2014-08-20
  • Contact: Liu Yi, Professor, Chief physician, Master’s supervisor, Military Center for Burn and Plastic Surgery, Lanzhou General Hospital of Lanzhou Military Region, Lanzhou 730050, Gansu Province, China
  • About author:Wang Gang, Physician, Second Clinical College of Lanzhou University, Lanzhou 730030, Gansu Province, China
  • Supported by:

    the Military Medical Science Research during the Twelfth Five-Year Plan, No. BWS11C061

PDF

885

被引次数

4

摘要：

背景：京尼平的低毒性具有一定的种属和细胞特异性，人脂肪间充质干细胞与京尼平交联的Ⅰ型胶原蛋白支架材料的生物相容性对于应用两者构建组织工程脂肪至关重要。

目的：评估人脂肪间充质干细胞与京尼平交联的Ⅰ型胶原蛋白支架材料的生物相容性。
方法：分离培养人脂肪间充质干细胞，传代培养至第3代，接种于京尼平交联的Ⅰ型胶原蛋白支架材料上。MTT法评估人脂肪间充质干细胞在支架材料上的黏附和增殖情况，支架材料对细胞的毒性作用；光镜和电镜分别观察人脂肪间充质干细胞在支架材料上的黏附和生长过程，以及细胞形态学变化。

结果与结论：人脂肪间充质干细胞接种于支架材料后，能够迅速在材料上黏附、增殖，其黏附率平均为86.5%；光镜和扫描电镜显示人脂肪间充质干细胞在支架材料上黏附性良好，随着时间的推移，细胞逐渐增多，可以迁移进入支架内部并均匀分布。结果表明京尼平对细胞的毒性低，交联后的支架材料与人脂肪间充质干细胞具有良好的体外生物相容性。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 材料相容性, 京尼平, 人脂肪间充质干细胞, Ⅰ型胶原蛋白, 生物相容性, 脂肪组织工程

Abstract:

BACKGROUND: Low toxicity of Genipin has certain species and cell specificity. Biocompatibility of Genipin cross-linked type I collagen with human adipose-derived stem cells is essential for construction of tissue-engineered adipose.

OBJECTIVE: To investigate the bbiocompatibility of Genipin cross-linked type I collagen with human adipose-derived stem cells.
METHODS: Human adipose-derived stem cells were isolated and cultured to the third generation, and the cells were seeded on Genipin cross-linked type I collagen scaffold. MTT assay was used to evaluate the adhesion and proliferation of cells on the scaffold, and the toxic effects of Genipin cross-linked type I collagen on human adipose-derived stem cells. Optical microscopy and scanning electron microscopy were utilized to observe the adhesion and growth process of human adipose-derived stem cells on the scaffold as well as the morphological changes of cells.
RESULTS AND CONCLUSION: Human adipose-derived stem cells could adhere to the scaffold immediately after seeded and increase gradually on the scaffold, with the average adhesion rate of 86.5%. Optical microscopy and scanning electron microscopy showed that human adipose-derived stem cells adhered well on the scaffold. The cells increased gradually over time, and could migrate into the scaffold, and distribute evenly with the passage of time when observed with optical microscopy. The result showed Genipin possesses very low cytotoxicity to the cells, and the outstanding biocompatibility is found between the cells and scaffold in vitro after cross-linked with Genipin.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: adipose tissue, mesenchymal stem cells, collagen type I, cross-linking reagents, biocompatible materials

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